Detection of S-Adenosyl-L-methionine in Fermentation Liquid by HPLC-UV
- VernacularTitle:HPLC-UV法定量测定发酵液中的S-腺苷-L-甲硫氨酸
- Author:
Heng CAI
;
Wei-Gang ZHENG
;
Hong-Gui WAN
;
Yi-Dan GUO
;
Tao WANG
;
- Publication Type:Journal Article
- Keywords:
S-Adenosyl-L-methionine HPLC UV
- From:
China Biotechnology
2006;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
A method for the determination of S-Adenosyl-L-methionine in fermentation liquid using the high performance liquid chromatography coupled with UV detector (HPLC-UV) was developed.A good linearity was obtained in the range of 0.1～1.0g/L (r = 0.9969) for S-Adenosyl-L-methionine.The average recoveries of S-Adenosyl-L-methionine were 99.89%～101.7% and the relative standard deviations were 0.48%～1.36%.The method is simple and rapid with reproducibility for the determination of S-Adenosyl-L-methionine in ferment liquid.
