Soluble sCD80-Linker-sCD40L fusion protein induces unspecific anti-tumor immunity in vitro
- VernacularTitle:可溶性sCD80-Linker-sCD40L融合蛋白诱导非特异性抗肿瘤免疫
- Author:
Dong XU
;
Feng WEI
;
Xiao-Da FU
;
Jin-Pu YU
;
Xiu-Bao REN
;
- Publication Type:Journal Article
- Keywords:
CD80;
CD40L;
sCD80-Linker-sCD40L;
fusion protein;
dendritic cells;
ovarian cancer cells;
anti-tumor immunity
- From:
Chinese Journal of Cancer Biotherapy
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the influence of sCD80-Linker-sCD40L fusion protein on the unspecific anti- tumor immunity in vitro.Methods:Ovarian cancer SKOV3 cells were separately transfected with recombinant adenoviral vectors containing sCD80-Linker-sCD40L fusion gene,sCD80 gene,sCD40L gene or with control adenovirus.The expres- sion of the sCD80-Linker-sCD40L fusion protein,sCD80 protein and sCD40L protein in the supernatants of SKOV3 cells was determined by ELISA.Dendritic cells(DCs)were cultured with peripheral blood mononuclear cells from a patient with ovarian carcinoma.DCs and autologous T cells were co-cuhured and were exposed to different supernatants for 48 h. The allostimulatory effects of DCs on T cells were determined by mixed lymphocyte reaction(MLR).The unspecific kill- ing activities of induced T cells against SKOV3/K562 cells were measured by LDH-releasing assay.Results:ELISA assay showed that levels of the sCD80-Linker-sCD40L fusion protein,sCD80 protein and sCD40L protein in the supernatants of transfeced SKOV3 cells were 2.791 ng/ml,1.956 ng/ml and 1.407 ng/ml,respectively.The fusion protein-exposed DCs ([0.382?0.053]vs[0.167?0.028],P
