Inhibitory effect of TNF-?on expression of hTERT and mdr1 gene in leukemic K562 and K562/ADM cell lines
- VernacularTitle:TNF-?对白血病K562及K562/ADM细胞hTERT及mdr1表达的抑制作用
- Author:
Li-Hua JIANG
;
Jing-Xue CHU
;
Shan-Hui SUN
;
Yang LIU
;
Ping HUANG
;
- Publication Type:Journal Article
- Keywords:
human telomerase reverse transcriptase;
tumor necrosis factor-or;
Leukemia
- From:
Chinese Journal of Cancer Biotherapy
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the inhibitory effect of TNF-?on hTERT gene expression in human myelogenous leukemia cell line K562 and K562/ADM and to study the influence of changed telomerase activity on expression of multi- drug resistance-1(mdr 1)gene.Methods:K562 and K562/ADM cells were treated with 5?10~6 U/L TNF-?for 24 h, then cell proliferation was detected by MTT assay and cell apoptosis was detected by flow cytometry.The expression of hTERT and mdrl mRNA was detected by RT-PCR and the telomerase activity was detected by ELISA.Results:TNF-?in- hibited the growth of K562 and K562/ADM cells and the inhibition showed a time-effect relationship(P
