Concise Plasmids for Antibiotic Resistance Cassette Recovery and in Vitro Recombination
- VernacularTitle:便于抗性基因分离和分子重组的系列质粒的构建
- Author:
Da-Wei LIU
;
Dan-Dan NIU
;
Liang ZHANG
;
Gui-Yang SHI
;
Zheng-Xiang WANG
;
- Publication Type:Journal Article
- Keywords:
Selectable marker, Antibiotic, Recovery
- From:
Microbiology
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
In gene manipulation, different selectable markers with various linkers are necessary. In order to get selectable markers directly, we constructed from pBlueScript SK(-) a series of particular plasmids, pSKsymKm, pSKsymBle, pSKsymEry, pSKsymHyg and pSKsymGm, each contains Kanamycin, Bleomycin, Erythromycin, Hygromycin or Gentamycin resistance cassette. By restriction enzyme digestion and gel extraction, any of five antibiotic resistance genes with specific ends can be conveniently obtained.
