Enzymatic Synthesis of D-D4FC Using Intact Cells
- VernacularTitle:整细胞酶促合成抗HIV新药D-D4FC
- Author:
Na QI
;
Li-Min ZHU
;
- Publication Type:Journal Article
- Keywords:
Intact cell,Enzymatic catalysis,D-D4FC,Anti-HIV,Synthesis
- From:
Microbiology
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
D-D4FC (?-D-2′,3′-didehydro-2′,3′-dideoxy-5-fluorocytidine),a new anti-HIV drug,is on its PhaseⅡ clinical trials in America,France and Germany. Our lab has synthesized D-D4FC successfully using N-deoxyribosyltransferase from Lactobacillus helveticto catalyzing the ribose transfer from D4T (?-D-2′,3′-unsaturated thymidine) to 5-FC (5-fluorocytidine).The yield of D-D4FC reached 25%.We discovered the reaction could also be done by using intact cells.The yield could increase to 50% in 12.5 hours and more convenient to industrial continuous process.In this paper,the conditions including pH,buffer,substrates concentration,cells amount,reaction time and a possible catalytic mechanism were studied and discussed.
