The Metabolic Control Breeding of L-Lactic Acid Fermentation and Optimization of Media and Cultivation Conditions
- VernacularTitle:L-乳酸发酵的代谢调控育种及发酵影响因素的研究
- Author:
Jun-Peng QIU
;
Yan XU
;
Wen-Quan RUAN
;
Qun YAN
;
- Publication Type:Journal Article
- Keywords:
L-lactic acid, Breeding, Optimization, Strategy of temperature control
- From:
Microbiology
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Based on the strain breeding theory and metabolic engineering theory, A high-yield mutant of Lactobacillus Thermophilus ATCC8317 was obtained through the compound inducements by the original Acetic acid-Sodium acetate plate and the productivity increased 210%.The best media components included saccharifying corn,malt powder 30g/L,peptone 5g/L.Based on the variety of specific cell growth rate and specific L-lactic acid production rate at different temperatures, the strategy of temperature control was obtained. The total product of L-lactic acid reached 135g/L besides the rate of glucose consumed and the average L-lactic acid productivity were up to 95% and 2.25g/(L?h) respectively.
