Study on the Extracting Method and Producing Conditions of Phyllosticta commelimecola Toxin
- VernacularTitle:鸭跖草叶点霉粗毒素的提取方法和产毒条件的研究
- Author:
Zu-Min GU
;
Ming-Shan JI
;
Xiu-Hua HAN
;
Song-Hong WEI
;
Ying-Zi WANG
;
- Publication Type:Journal Article
- Keywords:
Phyllosticta commelimecola Young, Toxin, Extraction, Toxin-producing condition
- From:
Microbiology
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
The crude toxin was extracted from hypha and culture solution of Phyllosticta commelimecola through three different polarity solvent: benzinum, puncificatum ethyl acetate and chloroform. The result indicated that the toxin secreted by Phyllosticta commelimecola not only was in hypha but also in culture solution and the extracting effect of ethyl acetate was the best. The soybean median and PSK media can be respectively used as solid and liquid culture media to produce toxin and grow mycelium. The optimal cultural conditions for producing toxin were temperature 32℃,cultured period 14d, cultured ways shaking of 150r/min.
