Cloning,High Level Expression and Purification of Human Survivin
- VernacularTitle:凋亡抑制因子Survivin的克隆、高效表达与纯化
- Author:
Hai LI
;
Yu PENG
;
Xiao-Tian LI
;
Wen-Yan WU
;
- Publication Type:Journal Article
- Keywords:
Survivin Clone Prokaryotic expression Purification
- From:
China Biotechnology
2006;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Survivin is a protein that inhibits apoptosis and regulates cell division.The cDNA sequence of survivin was amplified by RT-PCR and sub-cloned into the prokaryotic expression vector pET-21b(+),followed by transformation into E.coli strain BL21(DE3) and induction with IPTG.The recombinant survivin protein fusing with 6?His tag was expressed in E.coli in the form of inclusion body at the expression level over 60% of the total cell protein.Results of Western blotting showed that recombinant survivin reacted specifically with anti-human survivin antibody.After gel filtration,the recombinant protein reached the purity over 95%,which facilitate the study of diagnosing and inhibitor agents targeting survivin.
