Cloning for Full-length Pyruvate Orthophosphate Dikinase Gene of Sugarcane
- VernacularTitle:甘蔗全长丙酮酸磷酸二激酶(PPDK)基因的克隆
- Author:
Fu-Ming YANG
;
De-Chang XU
;
Ai-Ju HOU
;
- Publication Type:Journal Article
- Keywords:
Sugarcane PPDK gene LA-PCR technology Full-length gene clone
- From:
China Biotechnology
2006;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Compared with C3 plant,C4 plant had evident growth advantage,higher rates of water and nutrition using,and higher bio-yield than C3 plant,Sugarcane was one of the typical C4 plants.DNA was extracted from sugarcane leaves,primers were designed by the cDNA sequence of PPDK gene from GenBank.Then DNA was amplified by LA-PCR(Long Acute PCR) method,ligated into pMD18-T vectors,and transformed into E.coli.JM109.Full-length PPDK gene sequence of sugarcane was obtained,the sequence was 13.5kb in length.For convenient of the next experiment,two digest sites(XhoI and NotI) were introduced into primers,the full-length PPDK gene was splitted to two parts,and was ligated into pMD18-T simple vectors,respectively.Whole PPDK gene clone of sugarcane was finished.The strains were storaged in the lab.
