Establishment of simultaneous rapid genotyping of two mutation sites in the promoter region of interleukin 6 gene
- VernacularTitle:白细胞介素6基因启动子区两个突变位点多态性检测方法的建立
- Author:
Xing-Wang JIA
;
Ya-Ping TIAN
;
Xin-Xim DENG
;
- Publication Type:Journal Article
- Keywords:
Interleukin6;
Polymorphism,single nucleotide;
Polymerase chain reaction
- From:
Chinese Journal of Laboratory Medicine
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a rapid assay for genotyping of IL-6-597G/A and-572G/C polymorphisms by duplex real-time PCR assay.Methods One pair of primers and two pairs of fluorescent hybridization probes had been used to genotype two mutation sites in the promoter region of IL-6 gene by fluorescent resonance energy transfer and melting curves.Results Duplex real-time PCR method for genotyping of two mutation sites simultaneously had been developed and 123 health people samples were analyzed by this new method.The results showed that three genotypes were found in IL-6 gene-572G/C polymorphisms.They were GG,GC and CC genotypes.IL-6 gene promoter-597G/A polymorphism analysis showed 4 cases displayed GA genotype and other possessed GG genotype.No AA genotype had been found.Conclusions Duplex real-time PCR method is simple and fast.It provided an accurate and economic method which is suitable for clinical gene genotyping.
