Clinical study on change of chemokine mRNA and protein in patients with diabetes mellitus complicated infection
- VernacularTitle:糖尿病并发感染时趋化因子mRNA与蛋白表达变化的临床研究
- Author:
Jian GUO
;
Da-Lin SONG
;
Wei-Qiang KANG
;
- Publication Type:Journal Article
- Keywords:
Diabetes Mellitus;
Infection;
Cytokine;
Polymerase chain reaction
- From:
Chinese Journal of Laboratory Medicine
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate change of chemokine mRNA and protein in the patients with diabetes mellitus(DM)complicated infection.Methods Fourty-eight patients with DM complicated infection were studied.Expression of mRNA and protein of monocyte chemoattractant protein-1(MCP-1)and interleukin 8 (IL-8)were measured by FQ-PCR,RT-PCR and ELISA.The relationship between hs-CRP,PMNL,IL-8 and MCP-1 were analyzed.Results Expression of mRNA and protein of MCP-1 and IL-8 were increased in the DM patients complicated infection.MCP-1 mRNA(mesured by FQ-PCR)was positively correlated with the protein(IL-8 r = 0.33;MCP-1 r = 0.88;P
