The Application of Yeast One Hybrid System in Plant Transcription Factor Gene Resistant to Osmotic Stress
- VernacularTitle:酵母单杂交系统在植物抗渗透胁迫转录因子研究中的应用
- Author:
Qi WANG
;
Yanming ZHU
;
Dongdong WANG
;
- Publication Type:Journal Article
- Keywords:
Yeast one hybrid system Plant Osmotic stress Transcription factor DREB
- From:
China Biotechnology
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
The yeast one hybrid system is the new type system deriving from the yeast two hybrid system to study the interaction between DNA and protein.The principle and the technical line of this system was briefly introduced,and the progress of this system on the research of plant transcription factor gene resistant to osmotic stress was particularly summarized,such as cloning the transcription factor genes of osmosis resistance type;confirming the interaction between known DNA and protein;making sure the localization of the confirmed DNA binding domain with the interaction;also validating the transcription activation of the transcription factor gene.Furthermore,the problems existing in this system on the research of plant transcription factor gene resistant to osmotic stress was analyzed,and the development prospect according to the research was forecasted.
