Technologies for Surface Display of Lipase
- VernacularTitle:脂肪酶表面展示技术
- Author:
Wenshan LIU
;
Yunjun YAN
;
- Publication Type:Journal Article
- Keywords:
Lipase Surface display High throughput screen wholecell biocatalyst
- From:
China Biotechnology
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Lipase is a kind of widely used hydrolase.Surface display is an efficient method of highthroughput screening for protein engineering of lipase.Besides,lipase displaying on surface of microorganism has many advantages,such as higher stability against high temperature and organic solvent,compared with free lipase,so the host strain displaying lipase can be used as wholecell biocatalyst,which has some advantages compared with traditional immobilization of lipase.There are three kinds of host strain for displaying lipase:phage,bacteria and yeast.The surface display of lipase in the three display systems were systematically discribed,and their current uses and possible trends in the future were discussed also.
