Expression and Antigenic Analysis of the Recombinant Epitope of Herpes Simplex Virus Type 2 Glycoprotein G
- VernacularTitle:单纯疱疹病毒II型糖蛋白G主要抗原表位的表达和抗原性检测
- Author:
Xiao-Hong WANG
;
Hai-Rong LU
;
Gang ZHANG
;
Shao-Juan CHEN
;
De-Xin HUANG
;
Ling-Yun LI
;
Feng LIN
;
- Publication Type:Journal Article
- Keywords:
Human herpes simplex virus type 2 Glycoprotein G Antigenic epitope
- From:
China Biotechnology
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
A fragment containing amino acid residues 561~578 of HSV-2 glycoprotein G(gG2) was obtained by PCR assembling technique,and doubly cloned into vector pET-KDO.The recombinant plasmid was transformed to BL21(DE3)plysS.Fusion protein,of molecular weight about 39kDa was highly expressed by induction of IPTG.Western blot result showed the fusion protein had good antigenicity.After putification and digestion,the purity reached 95%.The digested purified protein was analysed by ELISA and showed good sensitivity and specificity.The recombinant protein should be useful for type-specific serodiagnosis of HSV-2.