Establishment and evaluation of hematology review criteria for action following automated complete blood counts and leukocytes differential
- VernacularTitle:自动全血细胞分析和白细胞分类复检规则的制定及评价
- Author:
Hong JIANG
;
Ting-Ting ZENG
;
Su-Gen ZENG
;
Man-Ying GUO
;
Xin-Qin ZHU
;
Yong-Tai ZUO
;
Yi LI
;
Yong-Qian JIA
;
- Publication Type:Journal Article
- Keywords:
Blood cell count;
Hematology analyzer;
Microscopy
- From:
Chinese Journal of Laboratory Medicine
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish and evaluate criteria applied to review of complete blood counts (CBC)and differential results from automated hematology analyzers.Methods Temporary criteria were established by using alarm system of XE-2100 automated hematology analyzer and by consulting the 41 suggested rides of international consensus group.2 795 out-and in-patient samples were run as clinical samples.Stained blood films were prepared and manual differential with smear review were performed on all samples.Statistical analysis was done for each temporary rule and instrument flag which indicated abnormal cell quantity and morphology.Results Of all rules,instrument flags of ‘Immature Gran/Left Shift?’, ‘ Atypical Lympho?’and‘NRBC(nucleated red blood cell)?’showed most frequent false positive and false negative instrument flag.Evaluation on rnles about cell quantity change showed false positive and false negative rates were both low.Results of morphology evaluation showed that true positive rate was 17.44%, false positive rate was 15.82%,true negative rate was 63.49%,false negative rate was 3.25%.‘ Atypical Lymphocyte?’,‘Immature Gran?’and‘blast?’were the most frequent false positive flags.According to those results and clinicians opinions,our hematology review criteria for action following automated CBC and leukocytes differential was established.Conclusions The hematology review criteria have high true positive rate and low false negative rate.To clinical hematology laboratory using automated hematology analyzer,new criteria can reduce work load,bring lower false negative rate and higher work efficiency.
