Screening of the Microbe Producing Docosahexaenoic Acid from Seawater and Identification on a Strain of High Yield
- VernacularTitle:海水中DHA产生菌的筛选及一株高产菌的鉴定
- Author:
Yu-Qiao ZHAO
;
Yun-Jian DU
;
Li-Yan WANG
;
- Publication Type:Journal Article
- Keywords:
Torulopsis,Docosahexaenoic Acid,Screening,Identification
- From:
Microbiology
1992;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Microbes that produce Docosahexaenoic Acid were isolated from seawater. 160 strains capable of producing lipids were screened out using Sudan Black B dying method from 280 seawater samples. From 60 strains of microorganisms producing bigger lipid particles, 7 strains of them capable of producing lipids more than 8% were obtained with Soxhlet abstracting method in the first screening. In the secondary screening from 10 strains with high lipids yield, strain 7-3 capable of producing 15.9% lipids was obtained, in which the content of DHA(Docosahexaenoic Acid)is 45.2%. Strain 7-3 was identified as Brettanomyces based on its morphological properties, cultural characteristics, physiological and biochemical properties.