Effects of Different Media on Enriching and Screening Fungi Culture with the Abilities of Decolorizing Various Synthetic Dyes
- VernacularTitle:不同培养基对富集筛选脱色真菌菌群的效果比较
- Author:
Zhen-Jie JIA
;
Hui-Jun LI
;
Qing-Xiang YANG
;
Hao ZHANG
;
Jian-Jun CHEN
;
- Publication Type:Journal Article
- Keywords:
Fungi, Decolorization of dyes, Medium, Enzyme
- From:
Microbiology
1992;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
In this paper 3 different media (A,for yeast cultivation ; B, for laccase producing; D, for white rot fungi cultivation) were compared in enriching and screening decolorizing fungi culture using Reactive Black 5 (RB5) and Reactive Red (M-3BE) from the following three points: decolorization effects, abilities of producing enzymes and diversity of microbial community. 11 groups of fungi with obvious decolorization effects were obtained after enrichment for near one month. Among them, 6 groups came from medium D, the other two 3 groups from medium A and B, respectively. However, the 3 groups from medium A exhibited the highest microbial diversity and best decolorization results with 99.53% and 97.42% color removal rate of Reactive Red M-3BE and Acid Red. From them, 16 strains of fungi were isolated and primarily identified as Saprolegniaceae, Eurotiaceae (Monascus went), Erysiphaceae and Physodermataceae. Fungi groups from medium B and D exhibited a bit lower color removal rate of various dyes and only 3 and 2 isolates primarily classified as Saccharomycetaceae and Eurotiaceae (Penicillium) were obtained from them. Fungi cultures in medium A and B could produce lignin peroxidase, and those in medium D could be detected higher activity of laccase. All the fungal cultures exhibited very weak activity of manganese dependant peroxidase.
