Site-saturation Mutagenesis Technology and Its Application in Protein Engineering
- VernacularTitle:点饱和突变技术及其在蛋白质工程中的应用
- Author:
Su-Dan TAO
;
Jia LIU
;
Xi-Wen CHEN
;
De-Fu CHEN
;
- Publication Type:Journal Article
- Keywords:
Site-saturation mutagenesis Protein engineering Target residues Overlap extension Beneficial mutant
- From:
China Biotechnology
2006;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Site-saturation mutagenesis is a newly-developed technology in protein engineering.By manipulating the encoding genes,it can rapidly obtain the mutants of desired proteins whose target residues are substituted by 19 other common amino acids.Site-saturation mutagenesis could serve not only as a powerful tool in protein engineering,but also as an important method in exploring the structure-function relationship of proteins.Several techniques were summarized to achieve site-saturation mutagenesis and introduce their application status in the protein engineering.The problem and promising future of its application were also discussed.
