A molecular epidemiological analysis of methicillin-resistant Staphylococcus aureus chromosomal cassette mec types with multiplex PCR strategy
- VernacularTitle:多重PCR法对耐甲氧西林金黄色葡萄球菌染色体mec盒的分型研究
- Author:
Bang-Lao XU
;
Ge ZHANG
;
Wei MA
;
Hui-Fen YE
;
Sui-De DENG
;
Pin-Hong BAO
;
Rong WANG
;
Wu-Bin PAN
;
Xi-Mei ZHAN
- Publication Type:Journal Article
- Keywords:
Polymerase chain reaction;
Staphylococcus aureus;
Genotype
- From:
Chinese Journal of Laboratory Medicine
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the genotypes of 102 strains of methicillin-resistant Staphylococcus aureus(MRSA)collected consecutively in 2002 in our hospital Method Multiplex PCR was used to genotype Staphylococcal chromosomal cassette mec(SCCmec)element and its variants.Results Among 102 strains of MRSA,the genotypes were as follows:SCCmec-Ⅲ(94 strains),SCCmec-ⅢA(4 strains), SCCmec-Ⅳ(2 stains),SCCmec-Ⅰ(2 stains).Conclusion The predominant genotype of MRSA circulating in this hospital in 2002 was SCCmec-Ⅲ by multiplex PCR.
