Detection of mitochondrial membrane potential changes in Myelodysplastic syndrome by fluorescent probe JC-1
- VernacularTitle:荧光探针JC-1检测骨髓增生异常综合征细胞凋亡早期线粒体膜电位的变化
- Author:
Guo-Hua XIA
;
Bao-An CHEN
;
Hui-Xia LU
;
Ze-Ye SHAO
;
Jia-Hua DING
;
Chong GAO
;
- Publication Type:Journal Article
- Keywords:
Myelodysplastic syndrome;
Mitochondria;
2-Methoxyestradiol
- From:
Chinese Journal of Laboratory Medicine
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the function of fluorescent probe JC-1 in detecting the changes of mitochondrial membrane potential(△?m)in early apoptotic cells.Methods After 2-ME was used to induce MUTZ-1 cell apoptosis,cells were dyed with fluorescent probe JC-1,and then the changes of △?m in the early stage of apoptotic cells were analyzed by flow cytometry or detected under fluorescent microscope. Results The control cells with high △?m are those forming JC-1 aggregates in the inner membrane of mitochondria,thus showing orange-red fluorescence.2-ME caused decrease of △?m in MUTZ-1 cells,in which JC-1 maintains monomeric form,thus showing only green fluorescence.The decreases of △?m were in a time-dependent manner,which were significantly higher than those in control group(P
