Optimization of Separating Conditions of Mouse Gut Flora by High Performance Ion Exchange Chromatography Analysis
- VernacularTitle:小鼠肠道菌群HPLC分析中色谱分离条件的优化
- Author:
Wen CHEN
;
Liang GUI
;
Juan LIN
;
Zhang-Jie CHEN
;
Shu-Tao LIU
;
Ping-Fan RAO
;
- Publication Type:Journal Article
- Keywords:
Mouse Gut flora High performance ion exchange chromatography Chromatographic conditions Optimization
- From:
China Biotechnology
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
The influence of separating effect of different chromatographic conditions of mouse gut flora by high performance ion exchange chromatography analysis was studied. The optimum chromatographic conditions for separating gut bacteria were determined. The sample was applied to the chromatography column packed with Toyopearl SuperQ-650c anion resin, equilibrated with 0.02mol/L piperazin-hydrochloric acid buffer (pH 8.0), and elution salt 1mol/L NaCl, eluted with the gradient of 0-50% NaCl/ 80 min, then 50%~75% NaCl/ 25 min at the flow rate 1ml/min, and injecting volume was 1ml.Under these conditions, intestinal flora were separated into several fractions. The establishment of HPLC analysis method will lay a foundation of further research on the components of mouse gut flora and their dynamic changes.
