Using Uniform Design to Optimize Expressive Conditions of rPA(K) in E.coli
- VernacularTitle:运用均匀设计法优化rPA(K)在原核系统中的表达条件
- Author:
Hui-Xia LUO
;
Ming LI
;
Yu-Jiong WANG
;
Chung-Ji MA
;
- Publication Type:Journal Article
- Keywords:
rPA(K) Uniform design Optimize expression
- From:
China Biotechnology
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Using uniform design to optimize some relative factors influencing the expressing of t-PA variant-rPA(K) in E.coli system, and found the best expressive conditions. They were(by using 300ml culture fask): the volume of media was 25ml, inducing time was 5.3 hours, pH was 6.0, the concentration of IPTG was 0.1mmol/L, inducing time was 25℃, and best culture media was HD. After being optimized, the yield of expression had been improved from 0.16 to 0.48, and it was as 3 times as before. The results above will offer the basement for purification and renaturation of rPA(K).
