Analysis of DNA fingerprint of Mycobacterium tuberculosis enterbacterial repetitive intergenic consensus-polymerase chain reaction
- VernacularTitle:肠杆菌基因间重复序列PCR指纹图谱技术分析结核分枝杆菌流行情况
- Author:
De-Cui PEI
;
Qing-Hua LUO
;
Xiang WANG
;
Shu-Lan WANG
;
Ya WANG
;
Jin-Yong WANG
;
- Publication Type:Journal Article
- Keywords:
Mycobacterium tuberculosis;
Repetitive sequences,nucleic acid;
Polymerase chain reaction;
DNA fingerprinting
- From:
Chinese Journal of Laboratory Medicine
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the epidemiological characteristics of Mycobacterium tuberculosis by enterbaeterial repetitive intergenic consensus-polymerase chain reaction(ERIC-PCR)DNA fingerprint. Methods Mycobacterium tuberculosis positive sputum samples between September 2003 to May 2006 were collected and cultured.Chromosomal DNA were extracted and ERIC-PCR DNA fingerprinting was analyzed by software,such as RAPD PHYLIP and Treeview.Results A total of 42 different fingerprints were detected.Phylogenetic analysis showed that they could be classified into three clusters,the clustering rate was 72.6%.The characteristics of ERIC-PCR fingerprint patterns were related to age,drug resistance,and type of resistance.Conclusions ERIC-PCR DNA fingerprinting technique used in this study is good for epidemiological studies with its strong discrimination,simplicity and rapidness.A high level of recent transmission is found in our city.
