The capacity of MTD method for distinguishing Mycobacterium tuberculosis and nontuberculosis mycobacteria
- VernacularTitle:应用MTD试剂盒对结核分枝杆菌复合群进行快速检测的评价
- Author:
Xiao-Hong GUI
;
Jian MEI
;
Yi-Feng WANG
;
- Publication Type:Journal Article
- Keywords:
Mycobacterium tuberculosis;
RNA probes;
Nucleic acid amplification technique
- From:
Chinese Journal of Laboratory Medicine
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the capacity of MTD method to distinguish between Myeobacterium tuberculosis and nontuberculosis mycobacteria.Methods Ten standard strains(including 1 H_(37)Rv strain and 9 nontuberculosis mycobacteria strains),94 clinical strains(including 48 Mycobacterium tuberculosis and 46 nontuberculosis mycobaeteria strains)and 40 sputum specimens were tested by MTD method(AMPLIFIED-MTI))and traditional methods.The results of these methods were compared.Results For all Myeobacterium tuberculosis and nontuberculosis mycobacteria strains,the agreement of MTD method and traditional method was 100%.And the positive detectable rate for sputum samples was 65% that was obviously higher than that for the direct smear(5/40),concentration smear(10/ 40)or culture(5/40).Conclusion MTD is a rapid test for identification of Mycobacterium tuberculosis and nontuberculosis mycobaeteria with high sensitivity and specificity.
