Preparation and cell toxicity of a novel long-circulating paclitaxel-containing liposome
- VernacularTitle:新型长循环紫杉醇脂质体的制备及其细胞毒性
- Author:
Tianzhi JIANG
;
Shaoyou LIU
;
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2007;0(26):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To prepare a novel long-circulating paclitaxel-containing liposomes, evaluate its quality and study its toxicity to gastric cancer cell line BGC823. METHODS: From May to November in 2006, the experiment was carried out in the Laboratory of Chemistry, Kaili College and the Laboratory of Physics and Chemistry Department, Southeast Guizhou Center for Disease Control and Prevention.①The novel long-circulating paclitaxel-containing liposomes was prepared by ultrasonic film method. Encapsulation efficiency and stability of the paclitaxel liposomes were measured by RP-HPLC.②The paclitaxel liposomes were dispersed in phosphate buffer at the pH value of 6.5, at 4 ℃ for 30 days, and the change of drug loads was observed.③The proliferation of BGC823 cell at logarithmic phase was tested by MTT method under the paclitaxel liposomes concentrations of 5, 10, 15, 20 mg/L. Meanwhile the paclitaxel free drug was taken as controls. RESULTS: ①Encapsulation efficiency of this paclitaxel liposomes was high to 97.6% detected by RP-HPLC.②When the paclitaxel liposomes were stored in refrigerator of 4 ℃, there was no flocculent precipitation during one month. Drug loaded in liposome after 3, 8, 15, 22, 30 days of preservation was respectively 97.3%, 96.2%, 95.6%, 94.9%, and 94.2%. The drug leakage of the paclitaxel liposomes was only 6% one month after dispersing in this buffer.③The result of in vitro cell toxicity showed that, under the drug concentration of 5, 10, 15, 20 mg/L, the inhibition rate to BGC823 cell of paclitaxel liposomes was 33.46%, 44.15%, 51.94%, 68.64% respectively, where those of free paclitaxel was 35.24%, 48.37%, 57.49%, 74.84%. CONCLUSION: ①This high-encapsulated and stable long-circulating paclitaxel-containing liposomes is expected to provide a more effective paclitaxel preparation.②The inhibition rate to BGC823 cell of the paclitaxel liposomes is low to that of free paclitaxel at the same drug concentration, indicating that it can present the slow release of liposomes.
