The Substrate Specificity of Cyclic Imide Hydrolase Mutants
- VernacularTitle:突变型环酰亚胺水解酶的底物专一性研究
- Author:
Yun-Xia CHEN
;
Li-Xi NIU
;
Jing-Ming YUAN
;
Ya-Wei SHI
;
- Publication Type:Journal Article
- Keywords:
Cyclic imide hydrolase Mutants Kinetic parameters Substrate specificity
- From:
China Biotechnology
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
The effect of C-terminal region residues on the substrate specificity of a novel cyclic imide hydrolase (CIH), a recombinant cyclic imide hydrolase (CIH293), and its mutants deleted or substituted at C-terminus (CIH291, CIH290, KK292-293EE) was reported. The substrate specificity and kinetic parameters of the mutants were analyzed by both the spectrophotometric assay and high-performance liquid chromatography. Results show that the substrate specificity of mutants was not obviously changed, but slightly low for the affinity between the substrate and enzyme, compared with the wild-type enzyme, CIH293. In conclusion, the last three residues of CIH293 play an important role for the enzyme activity.
