Transgenic Tobacco Expressing the Crocus CSzCD Gene Showed the Production of Crocetin
- VernacularTitle:转基因烟草表达西红花CSzCD基因产生西红花酸
- Author:
Hai-Bo XIAO
;
Lan WANG
;
Chen YAN
;
Ying XU
;
Fang CHEN
;
Lin TANG
;
- Publication Type:Journal Article
- Keywords:
Saffron Crocetin Crocin Zeaxanthin cleavage dioxygenase Transgenic tobacco
- From:
China Biotechnology
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the feasibility to produce crocetin in tobacco plants.The coding region of zeaxanthin cleavage dioxygenase (CSzCD) gene from Crocus sativus L. was inserted into the downstream of the cauliflower mosaic virus (CaMV) 35S promoter of a binary vector pBI121, and integrated into the genome of Nicotiana tabacum L. Twenty-one transgenic lines were identified by genomic southern blotting analysis. Western blotting and HPLC analysis of the leaf extracts of transgenic tobacco showed that crocetin was produced in CSzCD gene-transformed plants, while no crocetin was found in the untransformed tobacco.
