The Biosynthesis of ?-arbutin by Xanthomona maltophilia BT-112
- VernacularTitle:嗜麦芽黄单胞菌游离细胞催化合成?-熊果苷
- Author:
Xiu-Peng WANG
;
Shu-Rong ZHANG
;
Chun-Qiao LIU
;
Chun-Ying LIU
;
Peng ZHANG
;
- Publication Type:Journal Article
- Keywords:
?-Arbutin,Xanthomona maltophilia,Fermentation,Biocatalyst
- From:
Microbiology
1992;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
?-arbutin is biosynthesized by whole cell method with Xanthomona maltophilia BT-112.The conditions for cell biosynthesized ?-arbutin are investigated as follows:temperature,25℃;concentration of hydroquinone,30mmol/L;mol ratio of sucrose and hydroquinone,20∶1;time course of ?-arbutin biosynthesis,45 hours;rotational speed,160r/min;concentration of Xanthomona maltophilia BT-112,85g/L;concentration of K-2HPO-4-KH-2PO-4 buffer solution,25mmol/L;pH of K-2HPO-4-KH-2PO-4 buffer solution,8.0.Under the above optimal conditions,the maximum of molar conversion yield based on the amount of hydroquinone supplied reaches 86.7%.
