Screening of High-pyruvate-producing Strain Using by TTC-CaCO_3 Complexes Medium
- VernacularTitle:TTC-CaCO_3复合平板法快速筛选丙酮酸高产菌株
- Author:
Peng-Pai ZHANG
;
Sheng-Yu YANG
;
Biao-Sheng LIN
;
Xiao-Bing HU
;
- Publication Type:Journal Article
- Keywords:
TTC-CaCO3 Complexes Medium,PYR,Screening
- From:
Microbiology
1992;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
It designs a way that can easily screen high-pyruvate-producing strain.It is a intelligently selected method which can highly improve the efficiency of strain screening.The principle can be described as the following:On the CaCO-3 medium,a transparent ring can be exhibited based on the reaction of PYR produced by the strain and CaCO-3 in the medium for pyruvate-calcium is a kind of soluble substance,it is obviously that the high-pyruvate-producing strain has a bigger dimension of the transparent ring.On the other hand,color reaction between TTC and ADH indicate the enzyme activities which have a proportional relation with color,our object strain is a weak-ADH-enzyme-activities type with a weak metabolic flux from PYR to alcohol.So the white color strain may be the right choice.
