Application of RAPD Molecular Marker in the Phylogenetic Analysis of Soy Sauce Strains
- VernacularTitle:RAPD分子标记在酱油生产菌系统发育分析中的应用
- Author:
Bin WANG
;
Li PAN
;
Yong GUO
;
- Publication Type:Journal Article
- Keywords:
RAPD molecular marker,Soy sauce strain,Phylogenetic analysis,Polymorphism
- From:
Microbiology
1992;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
RAPD analysis of eight soy sauce strains isolated from commercial soy sauce koji was done with random primers,using Aspergillus oryzae AS3.951 and Aspergillus sojae AS3.495 as controls.Six appropriate primers(Primer1,Primer5,Primer6,Primer7,Primer8,Primer9)for RAPD-PCR were screened from nine random primers,and repetitive experiments demonstrated that their RAPD-PCR polymorphic patterns were stable.There were usually 4～8 bands in their RADP-PCR patterns,where the number of the main bands was 1～4 and the secondary bands were abundant.The phylogenetic tree of these strains was reconstructed according to their RAPD-PCR patterns,and it basically corresponded to traditional morphological taxology,demonstrating that the application of RAPD molecular marker in the phylogenetic analysis of soy sauce strains was feasible.
