Biases and interlaboratory variations of gamma-glutamyltransferase measurements before and after calibration with a common human serum calibrator
- VernacularTitle:定值冰冻人血清校准前后?-谷氨酰转移酶室间测定结果调查
- Author:
Hui-Min JIA
;
Guo-Bin XU
;
Qing TONG
;
Qing-Tao WANG
;
Shu-Kui LI
;
Zhen-Kun HE
;
Tao WANG
;
Wan-Chun DAN
;
- Publication Type:Journal Article
- Keywords:
gamma-Glutamyltransferase;
Accuracy and comparability;
Calibration
- From:
Chinese Journal of Laboratory Medicine
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the accuracy and comparability of ?-glutamyltransferase (?- GT) measurement results on human serum samples and controI materials before and after calibration with a common human serum calibrator.Methods A human serum calibrator was prepared by pooling fresh serum aliquots and assigning a value for ?-GT with the IFCC reference method.The calibrator together with 5 human serum samples and 10 control samples were sent to 15 clinical laboratories and the sermn and control samples were measured with different analytical systems before and after a calibration with the calibrator.The results were analyzed for biases and interlaboratory variations.Results For the serum samples,the calibration resulted in reductions in biases from -9.0%～-14.2% to -0.8%～-7.9%,and in interlaboratory variations from 6.9%～11.6% to 2.8%～4.4%.No improvement was observed on the control samples.Conclusions Accuracy and comparability of serum ?-GT measurements can be improved by using a common human serum calibrator.Some control materials may not be commutable for human serum in ?-GT measurements.
