Effects of the Culture Method on the Construction of Dermal Substitutes in vitro
- VernacularTitle:培养方式对真皮组织体外构建的影响
- Author:
Yan-Na HE
;
Chun-Mei DING
;
Hua JIANG
;
Yan ZHOU
;
Wen-Song TAN
;
- Publication Type:Journal Article
- Keywords:
Tissue engineering Dermal Spinner flask
- From:
China Biotechnology
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Culture environment is the key factor in the construction of dermal skin.It was investigated that the effects of the culture methods,including the static culture and spinner flask culture,and stir speeds on the cells proliferation,metabolism and distribution within collagenchitosan sponges.A higher cell density and specific growth rate was obtained with spinner flask culture versus static culture,especially,the 80 r/min spinner flask culture.The cell distribution in dermal substitutes from stirred culture system was more uniform than static culture,as well as that with increase of stir speeds in spinner flask.In summary,the spinner flasks culture with proper stir speed shows promise for the construction of dermal substitutes in vitro.
