The Progress and Application of Recombinant PCR
- VernacularTitle:重组PCR技术研究进展和应用
- Author:
Hao WANG
;
Xian-Jiang KANG
;
Qi WANG
;
- Publication Type:Journal Article
- Keywords:
Recombinant PCR Splicing by overlapping extension Jumping PCR DNA shuffling Highthroughput screening
- From:
China Biotechnology
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Recombinant PCR applies to fulfill gene recombination by PCR thermal reaction.Over the twenty years,it has branched into three characteristic strategies:splicing by overlapping extension(SOE),jumping polymerase chain reaction(JPCR)and DNA shuffling.Recently,the technique aimed with exploiting natural source of different allele genes is developing up on simplification of experimental procedure,on trap for mutation and variation,and on highthroughput screening with technology of surface display and fluorescent probe.The recombinant PCR is increasesing value in broad range from biological basic research to bioengineering study.
