Expression,purification and identification of hepatitis E virus pb166-GST fusion protein
- VernacularTitle:戊型肝炎病毒pb166-GST融合蛋白的表达、纯化及鉴定
- Author:
Zheng-Lin WU
;
Lai-Zhi YANG
;
Ying HE
;
Xin-Jian ZHU
;
Run-Xiang WU
;
Xue-Dong LU
;
- Publication Type:Journal Article
- Keywords:
Hepatitis E Virus;
Recombinant fusion protein;
Escherichia coli
- From:
Chinese Journal of Laboratory Medicine
2001;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To express,purify and identify recombinant hepatitis E virus(HEV) pb166-GST fusion protein using GST gene fusion system and investigate its potential role in researching Hepatitis E diagnostic antigen field.Methods The recombinant E.coli BL21 performed by our own laboratory was used to induce the HEV pb166-GST expression with IPTG.The products were purified by BD Biosience GST purifying system.The specific expression was identified by SDS-PAGE and Western blot.The experiment conditions and results were described and analysed.Results The resolved HEV pb166-GST fusion protein on SDS-PAGE showed a major band at position of 43 kD.The expressed proteins had a single expected band after purify and the protein was recognized by anti-GST antibody on PVDF membrane.Conclusion The recombinant HEV pb166-GST fusion protein is expressed in recombinant E.coli BL21 efficiently in this way,and might be used as a candidate for diagnostic antigen of HEV.
