Controlled comparison of two supplemented aerobic and anaerobic blood culture bottles to detect simulated bacteremia specimens
- VernacularTitle:两种血培养系统的需氧与厌氧瓶对模拟菌血症标本检测能力的对比研究
- Author:
Yao WANG
;
Ying-Chun XU
;
Xiu-Li XIE
;
Hui WANG
;
Min-Jun CHEN
;
- Publication Type:Journal Article
- Keywords:
Culture media,conditioned;
Bacteria,aerobic;
Bacteria,anaerobic
- From:
Chinese Journal of Laboratory Medicine
2001;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare BACTEC Plus aerobic and anaerobic bottles with BacT/ALERT FA aerobic bottles and FN anaerobic bottles in the ability of detecting simulated bacteremia specimens.Methods The 202 pairs of specimens were composed of 5ml sterile blood and defined loads of microorganisms.112 pairs of specimens in them also contained defined doses of antibiotics to simulate the patients undertaking antibiotic therapy.Time-to-detect(TTD)and positive percentages were evaluated in four groups,including aerobic bottles detecting aerobic bacteria,anaerobic bottles detecting anaerobic bacteria and facultative anaerobic bacteria,and aerobic bottles contained antibiotics detecting aerobic bacteria.Results The positive percentages of two kinds of aerobic bottles were both 100%.For the specimens with bacterial concentration of 10~2 and 10~3 CFU/ml,average TTDs of BACTEC Plus aerobic bottles[(13.69?3.74)h,(11.54?2.87)h]were faster than those of BacT/ALERT FA bottles [(16.76?5.62)h,(14.47?4.30)h;t=-5.674,-7.294,P
