Clinical significance of A?_(1-42) antibody determination in Alzheimer's disease patients
- VernacularTitle:阿尔茨海默病患者A?_(42)抗体测定的临床意义
- Author:
Jun-Heng LIU
;
Shi-Yang PAN
;
Ai-Min LI
;
Jian-Yi ZHU
;
Ji-Cheng PAN
;
Zheng-Lin CHEN
;
Ming-Qing TONG
;
- Publication Type:Journal Article
- Keywords:
Antibody,monoclonal;
Alzheimer's disease;
?-amyloid
- From:
Chinese Journal of Laboratory Medicine
2001;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Clinical significance of using ELISA to determine ?-amyloid(A?)_(1-42) antibody levels in the sera of patients with Alzheimer's disease(AD).Methods 96 wells PVC plate was coated with A?_(1-42)peptide.Serum of AD patient was competing with mouse A?_(1-42)monoclonal antibody in this assay.The second antibody was horseradish peroxidase(HRP)conjugated goat anti-mouse IgG.Serum A?_(1-42)antibody levels were determined by ELISA.Results The sensitivity of this assay was about 1 ng/ml.The recovery rate of this test was between 96.5% and 104.7%.The residual A?_(1-42)antibody levels in human serum or horse serum after A?_(1-42)antibody was removed by absorption were less than 1 ng/ml. Serum A?_(1-42)antibody levels in 37 AD patients[(5.1?1.9)ng/ml]were remarkably lower than those in normal people[(12.6?3.3)ng/ml,P
