Construction of Recombinant Adenovirus Expression Vector of Human Sema4C Gene and Its Expression in Mouse Myoblasts Cell Line C2C12
- VernacularTitle:人Sema4C重组腺病毒载体的构建及其在小鼠成肌细胞系C2C12中的表达
- Author:
Hai-Tao WU
;
Shu-Hong LIU
;
Yan WU
;
Jun-Die FAN
;
Wen-Hong FAN
;
Ming FAN
;
- Publication Type:Journal Article
- Keywords:
Sema4C Adenovirus vector Myoblasts C2C12 Proliferation index
- From:
China Biotechnology
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
To generate recombinant adenovirus expression vector of human Sema4C gene and observe its expression in mouse myoblasts cell line C2C12 for ensuring easy access to investigate the role of Sema4C gene during myogenesis. The recombinant plasmid was packaged and amplified after being transfected in HEK293 cells through Lipofectamine. After infecting C2C12 myoblasts with recombinant adenovirus vector, the adenoviral infection efficiency was determined by confocal microscope which showed that the expression of green fluorescence could be detected at 12h and then reached peak at 24h after recombinant adenovirus infection. The infection efficiency was almost 100% confirmed by FACS examination. Detection of WB indicated that the expression of Sema4C in C2C12 of recombinant adenoviral infection group was significantly higher than that of the control group (P
