Isolation,Purification and Biological Activity of the Polysaccharides from Phellinus nigiricans
- VernacularTitle:黑盖木层孔菌发酵液多糖的分离纯化及活性研究
- Author:
Xia LI
;
Wen-Min TIAN
;
Ming HUANG
;
Shan CHEN
;
Li-Ping ZHANG
;
- Publication Type:Journal Article
- Keywords:
Phellinus nigiricans Polysaccharides Isolation and purification Murine splenic lymphocytes
- From:
China Biotechnology
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
The water-soluble polysaccharide PNM isolated from the submerged culture of Phellinus nigiricans by hot water extraction and ethanol precipitation, was purified by freeze thawing, methods of Sevag, and then fractionated by Sepharose CL-6B gel filtration chromatography, giving a polysaccharide fraction named as PNMⅠ. HPLC analysis showed that PNMⅠwas a homogeneous polysaccharide, with a weight-average molecular weight of 29 kDa. GC analysis indicated that PNMⅠis a heteropolysaccharide composed of mannose, galactose and glucose with a ratio of 0.35:0.44:1.00. Pharmaceutical experiments showed both PNM and PNMⅠ have a significant effect on the immunomodulatory activities of murine splenic lymphocytes in vitro. Also PNMⅠ could inhibit the growth of sarcoma 180 solid tumor in vivo.
