Study on Separation and Purification of Zwittermicin A and its Stability
- VernacularTitle:Zwittermicin A的分离纯化及其稳定性的初步研究
- Author:
Zhong-Xin LIU
;
Shou-Wen CHEN
;
Jin HE
;
Zi-Niu YU
;
- Publication Type:Journal Article
- Keywords:
Zwittermicin A,Separation and Purification,Ion exchange resin,HPLC-MS, stability
- From:
Microbiology
1992;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Zwittermicin A was purified by ion exchange resin and HPLC from supernatants of Bacillus thuringiensis.subsp.kurstaki strain D1-23 cultivation.2.89mg pure Zwittermicin A was acquired,proved by HPLC-MS.Results show that the optimized wash concentration of NH_ 4 H_ 2 PO_ 4 is 5mmol/L at first step.Next step CH_ 3 COONH_ 4 concentration is 30 mmol/L,the gradient pH is 8.0～9.5.Totally 93% Zwittermicin A can be reserved with ion exchange resin.The temperature and pH stability experiments show the half life of Zwittermicin A is 48.22 minutes in 100℃,and it is more stable in lower pH in pH 2.0～12.0.
