Study on the Breeding of Histidine Producing Mutant and Its Properties
- VernacularTitle:L-组氨酸产生菌的选育及其特性的初步研究
- Author:
Qing-Shan CHEN
;
Wei-Guo ZHANG
;
- Publication Type:Journal Article
- Keywords:
L-Histidine, Corynebacterium glutamicum,Transketolase,Fermentation
- From:
Microbiology
1992;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
A L-histidine producing mutant was derived from Corynebacterium glutamicum HZ4221(TRA R DCP R AMT R histidase - )by means of mutagenesis with N-methy-N′-nitro- N-nitrosoguanidine(NTG).Contrast to original strain,the amount of histidine accumulation reached to a level of 5.31g/L in a medium containing 80g/Lglucose and 30g/L ammonium sulfate after cultured for 72 hours; the transketolase activity reduced to a degree of 15.7%.The utilization of the carbon sources,genetic stability,effect of metal ions were also been investigated in this paper.
