Expression and Identification of LfcinB Gene in Pichia pastoris
- VernacularTitle:抗菌肽牛乳铁多肽素(LfcinB)在毕赤酵母中的表达及活性鉴定
- Author:
Jun-Bo YI
;
De-Xin HUANG
;
Ling-Yun LI
;
Feng LIN
;
- Publication Type:Journal Article
- Keywords:
Bovine lactoferricin,Gene,Expression vector,Pichia pastors yeast expression system
- From:
Microbiology
1992;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
In present study,bovine Lactoferricin was first secretly expressed in Pichia pastors yeast expression system.The synthesized LfcinB gene fragment was cloned into expression vector pPIC9K,and then obtained recombinant plasmid,designated as pPIC9K-LfcinB,was linearized and transformed into Pichia pastors strains SMD1168 by electroporation.The transformants were screened with Geneticin and multiply-copy colonies were harvested,in which LfcinB gene was verified to inserted into yeast chromosome stably.The positive recombinant Pichia strains were induced with methanol to express LfcinB in culture supernatant.It's expressive products has high activity of killing bacteria.We concluded that LfcinB gene was cloned and integrated into yeast chromosomes,and obtained expression peptide was tested to have high antibacterial activity.
