Direct spectrophotometric method to determing serum copper with a new water soluble reagent
- VernacularTitle:用水溶性显色剂直接光度法测定血清铜
- Author:
Tie-Li ZHOU
;
Chang-Rong ZHAO
;
Hong-Wu XIAO
;
Guo-Jun LIAN
;
Jian-Ming CAO
;
- Publication Type:Journal Article
- Keywords:
Serum copper;
Spectrophotometry;
2-(5-nitro-2-pyridylazo)-5-(N-propyl-N-sulfopropylamino)phenol
- From:
Chinese Journal of Laboratory Medicine
2001;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a simple and sensitive method for the determination of serum copper by spectrophotometry.Methods Nitro-PAPS was used as a coloring agent for serum copper in the presence of surfactants Tween-80 and Triton X-100 and the formed complex was measured by spectrophotometry.Results The maximum absorption wavelength of the complex was 570 nm and the molar absorption coefficient was 7.95?10~4 L/(mol?cm).The lineafity of the method was up to 63.0 ?mol/L and the recoveries ranged from 98.6% to 103.1%.The within-run and between-run CVs were 2.1%-3.3% and 2.7%-3.8%.The method(Y)was compared with an AAS method(X)and a correlation of Y=1.01X -0.27(r=0.998 2)was obtained.A reference interval(x~-?2s)determined with this method on 68 individuals was 9.7-24.1 ?moL/L.Conclusions A simple and sensitive method for serum copper has been established.It may used for the analysis of serum copper in clinical laboratories.
