Differential proteomic analysis of total protein of ASMC stimulated by wild and mutant IL-13
- VernacularTitle:白细胞介素13及变异体刺激呼吸道平滑肌后差异蛋白分析
- Author:
Xiao-Feng JIANG
;
Xiao-Ying GUO
;
Hong-Yan LIANG
;
Xue-Fei DU
;
Yu XIN
;
Li XUE
;
Song-Bin FU
;
- Publication Type:Journal Article
- Keywords:
Interleukin-13;
Myocytes smooth muscle;
Electrophoresis;
Mass spectrometry
- From:
Chinese Journal of Laboratory Medicine
2001;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the differential proteomics of ASMC stimulated by wild IL-13 and mutant IL-13 and to investigate the relations of protein profiles of ASMC to asthma and possible targets for the treatment of bronchial asthma.Methods The total proteins of ASMC stimulated by wild IL-13 and mutant IL-13 were separated by immobilized pH gradient(IPG)-based 2-DE and the differentially expressed protein spots were identified by matrix assisted laser desorption-time of flight mass spectrometry(MALDI-TOF-MS). Results The 2-DE detected approximately(840?21)spots on wild IL-13 samples and(892?17)spots on mutant IL-13 samples(n=3)and(685?19)spots matched.Six significantly differential proteins were subjected to MALDI-TOF-MS analysis and three of them were identified as stathmin 1,Ribosomal protein p~0 and NADH dehydrogenase.Conclusions ASMCs stimulated by wild IL-13 and mutant IL-13 present different proteomic profiles that may shed some light on the mechanism for the asthma causing effect of wild IL-13 and mutant IL-13.
