Expression of Human Interferon-?1 and Interferon-? Gene in WI-38 Cells and Comparison of Their Biological Activity
- VernacularTitle:人lambda1和epsilon干扰素基因在WI-38细胞中的表达及其活性比较(英文)
- Author:
Bhushan SARODE
;
Wei-Hua SHENG
;
Yu-Feng XIE
;
Yao-Dong ZHAO
;
Jing-Cheng MIAO
;
Ji-Cheng YANG
;
- Publication Type:Journal Article
- Keywords:
Lambda1 interferon Epsilon interferon WI-38 cell Antivirus Antiproliferation
- From:
China Biotechnology
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
The biological!activities i.e. antineoplastic activities and antiviral activity of the two novel kinds of interferons: hIFN-?1 and hIFN-? were studied and compared. First the fusion expression vector: pcDNA3.1A-hIFN-?1-His and pcDNA3.1A-hIFN-?-His by PCR was constructed,then the two kinds of plasmids were transfected into WI-38 (human embryonic lung cells) with liposome. And cytopathic effect (CPE) suppression test was used to study and compare the antiviral activities of rhIFN-?1-His and rhIFN-?-His, meanwhile MTT assay was used to detect their antineoplastic activities.It was found that, antiproliferative activity and MxA protein induction shown by rhIFN-?1-His is more powerful than of rhIFN-?-His. The antiviral molecular mechanisms of both hIFN-?1 and hIFN-? are related to MxA.The foundation for further study on the bioactivities and mechanism of action of hIFN-?1 and hIFN-? was established.
