Construction of an Inducible Expression and Secretion System for Neutral Protease in Bacillus subtilis
- VernacularTitle:中性蛋白酶基因诱导型表达分泌载体的构建
- Author:
Min ZHANG
;
Cong ZHAO
;
Lian-Xiang DU
;
Fu-Ping LU
;
Xing-Wang CAI
;
- Publication Type:Journal Article
- Keywords:
Bacillus subtilis Neutral protease Inducible expression
- From:
China Biotechnology
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
The promoter and signal peptide sequence of sacB gene (sacR gene) has been amplified by PCR.An inducible expression and secretion vector pHP13SN has been constructed with this amplified sequence,which was ligated with the pro-peptide and mature peptide of neutral protease gene on the vector pHP13.Transforming Bacillus subtilis DB104 with the vector pHP13SN, and the recombinant strain DB104(pHP13SN) can be got.The neutral protease gene has been expressed by the inducement of sucrose and the regulation of sacR,and the production has been secreted with bioactivity.
