Determination of serum cholesterol by isotope dilution gas chromatography mass spectrometry
- VernacularTitle:同位素稀释气相色谱质谱法测定血清总胆固醇
- Author:
Jiang-Tao ZHANG
;
Cui-Hua HU
;
Chuan-Bao ZHANG
;
Hai-Jian ZHAO
;
Rong MA
;
Jie-Hong XIE
;
Jing WANG
;
Jun DONG
;
Shu WANG
;
Ziyu SHEN
;
Wenxiang CHEN
;
- Publication Type:Journal Article
- Keywords:
Lipoproteins;
Cholesterol;
Gas Chromatography-Mass spectrometry
- From:
Chinese Journal of Laboratory Medicine
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a new isotope dilution gas chromatography mass spectrometry method (ID/GC/MS) for the measurement of serum cholesterol.Methods Serum was mixed with an isotope labeled internal standard ([3,4-~(13)C]-cholesterol) and treated with alcoholic sodium hydroxide to hydrolyze cholesterol ester to cholesterol.Cholesterol and internal standard was extracted and derived by N, O-Bis(trimethylsilyl) trifluoroacetamide to trimethylsilyl ethers.The derivation products were analyzed by capillary column GC combined with electron impact MS using scan and selected ion monitor (SIM) modes. Signals of cholesterol internal standard were corrected for the contributions from cholesterol and the signal ratio of cholesterol to internal standard for the calibrators were linearly regressed against cholesterol concentrations.The resulted regression equation was used for the calculation of serum cholesterol concentrations.Results The new ID/GC/MS method showed a mean within-run coefficient variance (CV) of 0.04%-0.81%.Comparison with two levels of standard reference material (SRM1951a) of National Institute of Standards and Technology (NIST) displayed a bias of 0.19% and 0.90% respectively.Conclusion A time-gaining ID/MS method has been established that is highly precise and accurate and can be used for the measurement of serum cholesterol.
