Examination of anti-HBx in sera from patients of chronic hepatitis B,liver cirrhosis and hepatocellular carcinoma and its clinical significance
- VernacularTitle:慢性乙型肝炎和肝硬化及肝癌患者血清中anti-HBx测定及其临床意义
- Author:
Hang ZHANG
;
Lian-Ying WU
;
Shu-Ye LIU
;
Xin-Hua SHAO
;
Zong-Wei YANG
;
Xiu-Qin ZHANG
;
Xue-Li GENG
;
Li-Hong YE
;
Xiao-Dong ZHANG
- Publication Type:Journal Article
- Keywords:
Hepatitis B virus;
Enzyme-linked immunosorbent assay ( ELISA );
Biological markers
- From:
Chinese Journal of Laboratory Medicine
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method of detecting hepatitis B virus x antigen (HBxAg) and antibody to HBxAg (anti-HBx) and to demonstrate its clinical significance of HBxAg and anti-HBx in sera from patients of chronic hepatitis B (CHB),liver cirrhosis (LC) and hepatocellular carcinoma (HCC). Methods Full length HBx gene was cloned into pET30a(+),a prokaryotic expression vector,named pET30a-X.It was transformed into Escherichia coli BL21 (DE3),followed the fusion protein of HBx-His was induced by IPTG.The purified fusion protein was used to immunize rabbit as an antigen to generate polyclonal antibody to HBx protein.The method of enzyme-linked immunosorbent assay (ELISA) was established by using purified fusion protein and generated antibody,which was used to detect HBxAg and anti-HBx in sera from patients of CHB,LC,HCC and normal healthy people.Results The positive rates of HBxAg/anti-HBx were 8.7%/10.4% for CHB,17.9%/40.6% for LC,and 9.8%/34.4% for HCC, respectively.In statistics,the positive rates of anti-HBx in LC and HCC were higher than that in CHB (P
