Determination of serum progesterone by isotope dilution gas chromatography mass spectrometry
- VernacularTitle:同位素稀释气相色谱质谱法测定血清孕酮
- Author:
Tian-Jiao ZHANG
;
Rui-Feng XU
;
Wei-Hua WANG
;
Xin-Hua DAI
;
Chuan-Bao ZHANG
;
Wen-Xiang CHEN
;
- Publication Type:Journal Article
- Keywords:
Progesterone;
Isotope dilution mass spectrometry;
2-Hydroxypropyl-?-cyclodextrin;
Gas chromatography
- From:
Chinese Journal of Laboratory Medicine
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a candidate reference method for the measurement of progesterone in human serum.Methods The serum sample is mixed with the internal standard [3,4-~(13)C_2] progesterone.After extraction with n-hexane and purified by a aqueous solution of 2-Hydroxypropyl-?- cyclodextrin (HP-?-CD),the serum progesterone and labeled progesterone are converted to the 3-enol heptafluorobutyrate and analyzed by gas chromatography mass spectrometry (GC/MS) with selected ion monitoring.The concentration of serum progesterone is calculated by bracketing method.Results The results gave coefficients of variation (CVs) of 0.69% to 2.12%.The analytical recoveries ranged from 98.3% to 100.1%.The results of measuring certified reference materials of serum progesterone are agree with the target value.Conclusion The procedure for measuring progesterone in serum is a highly accurate and precise method and may be used as a candidate reference method for serum progesterone assays.
