Study on Immune Efficacy of Single and Double Fusion DNA Vaccine from Mycobacterium bovis
- VernacularTitle:牛分枝杆菌单基因及双价融合基因DNA疫苗免疫效果的研究
- Author:
Qiang GONG
;
Si-Guo LIU
;
Chun-Lai WANG
;
Yong WANG
;
Jian-Dong LIU
;
Lei CHI
;
Kun ZHAO
;
Yuan-Yuan ZHOU
;
Yue-Hong CHANG
;
Mengke YUN
;
Xiangang KONG
;
- Publication Type:Journal Article
- Keywords:
Mycobacterium bovis Single gene Fusion gene DNA vaccine
- From:
China Biotechnology
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
The DNA fragments of ag85b、esat-6、hsp65、mpb64 and ag85b-esat-6、hsp65-esat-6、mpb64-esat-6 were amplified by PCR and SOE technique.These seven fragments were inserted into pCDNA3.1(+)vector to construct recombinant plasmids pCA、pCE6、pCH、pCM、pCAE、pCHE and pCME.The seven plasmids were transfected into SP2/0 cell in vitro to detect the expression of target genes.BALB/c mice were intramuscularly vaccinated with the seven plasmids and the control vector pCDNA3.1(+)and PBS respectively.The serum antibodies and the spleen lymphocyte proliferation(SLP)and secreted IFN～? of spleen were tested.The results of indirect ELISA showed the levels of antibodies in all recombinant plasmids groups were significantly higher than the two control groups(P
