The Influence of Two Resins on Synthesizing HCV Polypeptides by Solid-phase Method
- VernacularTitle:两种树脂对固相法合成丙型肝炎疫苗多肽的影响
- Author:
Li-Shu SUN
;
Hong-Kun TANG
;
Wu-Ling CHEN
;
- Publication Type:Journal Article
- Keywords:
Wang resin 2-chlorotrityl choloride resin Polypeptides
- From:
China Biotechnology
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
The solid-phase method to synthesis HCV polypetides was used.The connecting rate and the polypeptides' productivity and purity were compared when Wang resin and 2-chlorotrity choloride resin acting as vectors.By the identification of mass spectrum,the products' molecular weight was analysed.By ninhydrin method we compared to the connecting rate between two resins and amino acid.By RP-HPLC we carried out the analysis and purity of hetero peptides.The result showed the first amino acid's connecting rate along with the purity and productivity which we need when 2-chlorotrityl choloride resin acted as vector was notably higher than those when Wang resin acted as vector.So 2-chlorotrityl choloride as vector is used to synthesize HCV polypeptides,which adapts to the demand of big scale's industrialization production.
